Research Articles
Strategies for Overcoming Metabolic Burden in Engineered Microbial Hosts: From Foundational Concepts to Clinical Translation
Metabolic burden, a major bottleneck in developing robust microbial cell factories, arises from the rewiring of host metabolism for bioproduction, leading to impaired growth and low yields.
Beyond E. coli: Strategies for Predictable Genetic Circuit Design Across Diverse Microbial Chassis
This article addresses the central challenge in synthetic biology: the unpredictable performance of genetic circuits when transplanted between different microbial hosts.
Engineering Robust Microbial Cell Factories: Strategies for Enhanced Performance and Stability in Industrial Bioprocesses
This article provides a comprehensive analysis of contemporary strategies to enhance the robustness of microbial cell factories, a critical determinant for successful industrial-scale bioproduction.
Metabolic Pathway Reconstruction in Non-Model Organisms: A Comprehensive Guide from Theory to Clinical Application
The reconstruction of metabolic pathways in non-model organisms is a cornerstone of modern synthetic biology, enabling the development of novel microbial cell factories for drug discovery and biomanufacturing.
Beyond E. coli: Engineering Host-Agnostic Genetic Devices to Revolutionize Biomedicine
Host-agnostic genetic device engineering represents a paradigm shift in synthetic biology, moving beyond traditional model organisms to create genetic systems that function predictably across diverse microbial and mammalian hosts.
Building a Better Factory: Engineering Genome-Reduced Microbial Chassis for Heterologous Natural Product Production
This article provides a comprehensive resource for researchers and drug development professionals on the strategic deletion of native biosynthetic gene clusters (BGCs) to create optimized heterologous production chassis.
Dynamic Metabolic Control: Advanced Strategies to Decouple Cell Growth and Production for Biomedical Applications
This article explores dynamic metabolic control strategies that resolve the fundamental conflict between cell growth and product synthesis in engineered microbial systems.
Modular Vector Design for Broad-Host-Range Synthetic Biology: Expanding the Chassis Landscape for Biomedical Innovation
This article explores the paradigm shift in synthetic biology from a narrow focus on traditional model organisms to a broad-host-range approach that treats the microbial chassis as a central, tunable...
Streamlining Cellular Factories: A CRISPR/Cas9 Guide to Genome Reduction in Microbial Chassis Strains
This article provides a comprehensive overview of the application of CRISPR/Cas9 for genome reduction in industrial chassis strains, a key strategy in synthetic biology for enhancing microbial production hosts.
Transcription Factor Engineering for Enhanced Microbial Strain Tolerance: Strategies, Applications, and Future Directions
This comprehensive review explores transcription factor (TF) engineering as a powerful strategy to enhance microbial strain tolerance, a critical bottleneck in industrial biotechnology and bioprocessing.