Research Articles
Thermodynamic Feasibility Analysis of Cofactor Specificities: From Foundational Principles to Optimized Pathway Design
This article provides a comprehensive guide for researchers and scientists on integrating thermodynamic constraints into the analysis and engineering of metabolic pathways, with a specialized focus on cofactor specificity.
NADPH Oxidases: From Isoform-Specific Functions to Therapeutic Targeting in Disease
This article provides a comprehensive overview of the NADPH oxidase (NOX) family of enzymes, the only known enzymes whose primary function is reactive oxygen species (ROS) generation.
Cofactor Balance in Drug Discovery: Bridging In Silico Predictions and Experimental Validation
Cofactor balance is a critical determinant of success in metabolic engineering and drug discovery, influencing everything from cellular viability to product yield.
Cofactor Swapped Enzyme Variants: Performance Comparison, Engineering Strategies, and Biomedical Applications
This article provides a comprehensive comparison of cofactor-swapped enzyme variants, a critical protein engineering approach for optimizing metabolic pathways in biocatalysis and therapeutic development.
A Comprehensive Guide to Measuring Intracellular NADPH and ATP Levels Using HPLC: From Foundational Principles to Advanced Applications
Accurate quantification of intracellular NADPH and ATP is crucial for research in cellular metabolism, redox biology, and drug development.
Cofactor Regeneration Pathways: A Comparative Analysis of Efficiency and Applications in Biocatalysis and Drug Development
This article provides a comprehensive comparative analysis of cofactor regeneration pathways, essential for the economic viability of oxidoreductase-based biocatalysis.
Validating Cofactor Balance Using 13C Metabolic Flux Analysis: A Guide for Biomedical Research and Drug Development
This article provides a comprehensive guide for researchers and drug development professionals on applying 13C Metabolic Flux Analysis (13C-MFA) to validate cellular cofactor balance.
Static Pools vs. Dynamic Flux: Comparing NADPH Regulation Strategies in Metabolism and Disease
This article provides a comprehensive comparison of static and dynamic strategies for studying and modulating NADPH regulation, a central hub in cellular redox metabolism.
NADPH vs NADH: Decoding Enzyme Efficiency for Advanced Biocatalysis and Drug Development
This article provides a comprehensive analysis of NADPH and NADH-dependent enzymes, crucial cofactors with distinct yet interconnected roles in cellular metabolism.
Cofactor Swapping in Enzymes: Strategies for Enhancing Catalytic Efficiency and Biomedical Applications
This article provides a comprehensive overview of cofactor engineering as a powerful strategy to optimize enzyme catalytic efficiency, stability, and cofactor balance for biomedical and industrial applications.