Research Articles
Cofactor Economy: Pathway Engineering Strategies to Reduce NADPH Consumption and Boost Bioproduction
This article provides a comprehensive overview for researchers and drug development professionals on advanced strategies in metabolic engineering to reduce NADPH consumption.
Rational Engineering of NADPH and ATP Regeneration Pathways: Strategies for Enhanced Bioproduction and Therapeutic Innovation
This article synthesizes the latest advances in cofactor engineering, focusing on the rational redesign of NADPH and ATP regeneration pathways to power microbial cell factories and cellular processes.
NADPH Overdrive: How Pentose Phosphate Pathway Enzyme Overexpression Fuels Disease and Therapy
This article synthesizes current research on the overexpression of pentose phosphate pathway (PPP) enzymes as a central mechanism in metabolic reprogramming, driving NADPH production to support cancer proliferation, therapy resistance,...
Promoter Engineering for Enhanced NADPH Regeneration: Strategies for Bioproduction and Biomedical Applications
This comprehensive review explores promoter engineering as a pivotal metabolic engineering strategy to enhance NADPH regeneration, addressing a critical bottleneck in the microbial production of high-value pharmaceuticals and biochemicals.
Genetically Encoded ATP and NADPH Biosensors: Revolutionizing Dynamic Monitoring and Control in Metabolic Engineering
This article provides a comprehensive overview of the latest advances in genetically encoded biosensors for ATP and NADPH, crucial cofactors in cellular metabolism.
CSR-SALAD: A Structural Guide to Engineering Enzyme Cofactor Specificity for Metabolic and Drug Discovery
This article provides a comprehensive resource for researchers and drug development professionals on CSR-SALAD (Cofactor Specificity Reversal – Structural Analysis and Library Design), an automated computational tool for reversing enzyme...
Static Regulation Strategies for NADPH Regeneration: Foundational Methods and Advanced Applications in Metabolic Engineering
This article provides a comprehensive overview of static regulation strategies for NADPH regeneration, a critical cofactor in cellular antioxidative defense and reductive biosynthesis.
Dynamic Regulation of NADPH/NADP+ Balance: A Biosensor-Driven Approach for Metabolic Engineering and Drug Discovery
The NADPH/NADP+ redox couple is a central regulator of cellular redox homeostasis, antioxidant defense, and reductive biosynthesis.
Cofactor Engineering: The Key to Unlocking Robust Heterologous Protein Production for Therapeutics and Research
This article provides a comprehensive analysis of the critical link between intracellular cofactor availability and the efficiency of heterologous protein production.
Cofactor Swapping: Boosting Theoretical Product Yield in Metabolic Engineering and Drug Development
This article comprehensively explores the strategic rewiring of microbial metabolism through cofactor swapping—the engineering of enzymatic cofactor specificity from NAD(H) to NADP(H) or vice versa.