Research Articles
Microbial Cell Factories: Development, Applications, and Future in Sustainable Biomanufacturing
This article provides a comprehensive overview of the development of microbial cell factories (MCFs) for sustainable chemical and therapeutic production.
Synthetic Biology and Metabolic Engineering: Powering the Next Generation of Biomanufacturing and Therapeutics
This article provides a comprehensive overview for researchers and drug development professionals on the synergistic fields of synthetic biology and metabolic engineering.
MS vs NMR in Isotopic Labeling: A Comprehensive Guide for Biomedical Researchers
This article provides a comparative analysis of Mass Spectrometry (MS) and Nuclear Magnetic Resonance (NMR) spectroscopy for isotopic labeling studies, tailored for researchers and drug development professionals.
Validating Internal Flux Predictions in Flux Balance Analysis: From Foundational Concepts to Advanced Applications in Biomedicine
Accurately validating internal flux predictions is a critical challenge in Flux Balance Analysis (FBA), directly impacting its reliability in drug discovery, metabolic engineering, and systems biology.
Comparative Analysis of Objective Functions for Flux Prediction: From FBA to Machine Learning
This article provides a comprehensive comparative analysis of objective functions used in metabolic flux prediction, a critical task for researchers, scientists, and drug development professionals.
Quantifying Confidence in Metabolic Flux Analysis: From Foundational Concepts to Advanced Bayesian Methods
Accurate quantification of confidence intervals is crucial for interpreting metabolic flux estimates derived from stable isotope labeling experiments, yet the nonlinear nature of these systems presents significant statistical challenges.
Validating Genome-Scale Metabolic Models: A Robust Framework Using 13C Metabolic Flux Analysis
This article provides a comprehensive framework for evaluating the robustness of Genome-Scale Metabolic Model (GSMM) reconstructions using 13C labeling data.
Benchmarking Neural-Mechanistic Hybrid Models Against Traditional FBA: A New Paradigm for Predictive Metabolic Modeling
This article provides a comprehensive analysis for researchers and drug development professionals on the emerging paradigm of neural-mechanistic hybrid models and their benchmarking against traditional Flux Balance Analysis (FBA).
Metabolic Flux Analysis in Cancer Research: A Comparative Guide to Methods, Applications, and Best Practices
Metabolic reprogramming is a established hallmark of cancer, driving tumor progression and therapy resistance.
Beyond the χ²-Test: A Modern Guide to Goodness of Fit and Model Validation in 13C Metabolic Flux Analysis
This article provides a comprehensive guide to goodness of fit (GOF) testing and model validation for 13C Metabolic Flux Analysis (13C-MFA), a gold-standard technique for quantifying intracellular reaction rates.