Research Articles
Dynamic Control of Metabolic Pathways: Strategies to Reduce Toxic Intermediate Accumulation in Engineering and Therapeutics
This article explores the paradigm of dynamic control in metabolic pathways to mitigate the accumulation of toxic intermediates, a critical challenge in metabolic engineering and drug development.
Resolving Network Gaps in Genome-Scale Metabolic Reconstructions: A Comprehensive Guide from Theory to Clinical Application
Genome-scale metabolic models (GEMs) are powerful computational tools that predict cellular phenotypes from genomic information, but their predictive accuracy is often hampered by network gaps—missing reactions that disrupt metabolic pathways.
Balancing Act: Strategic Management of NADPH and ATP Cofactors in Engineered Metabolic Pathways for Biomedical Applications
This comprehensive review addresses the critical challenge of maintaining NADPH and ATP cofactor balance in engineered metabolic pathways, a fundamental requirement for efficient bioproduction in microbial cell factories and therapeutic...
Improving Metabolic Model Predictions: A Guide to Addressing Thermodynamic Feasibility
Thermodynamic feasibility is a critical but often overlooked constraint in genome-scale metabolic models (GEMs).
Engineering Yeast Cell Factories: A Comprehensive Guide to Heterologous Pathway Expression for Drug Development
This article provides researchers, scientists, and drug development professionals with a systematic framework for harnessing yeast cell factories for heterologous pathway expression.
High-Throughput Screening in Metabolic Engineering: Biosensors, Workflows, and Strain Optimization
This article provides a comprehensive overview of advanced high-throughput screening (HTS) methods that are revolutionizing metabolic engineering.
Advanced Enzyme Manipulation Strategies for Optimizing Metabolic Pathways in Drug Development
This article provides a comprehensive overview of contemporary enzyme manipulation strategies for optimizing metabolic pathways, tailored for researchers and professionals in drug development.
A Modern Guide to 13C Metabolic Flux Analysis: Protocol, Applications, and Best Practices
13C Metabolic Flux Analysis (13C-MFA) is the gold-standard technique for quantifying intracellular reaction rates in living cells, providing critical insights into cellular physiology for metabolic engineering, biotechnology, and disease research.
Dynamic Control of Metabolism: Engineering Genetic Circuits for Precision Microbial Factories and Therapeutics
This article explores the implementation of genetic circuits for dynamic metabolic control, a cutting-edge approach in synthetic biology that moves beyond static engineering.
Dynamic Metabolic Regulation: Advanced Biosensor Design for Pathway Optimization and Biomedical Applications
This article provides a comprehensive analysis of transcription factor-based and nucleic acid-based biosensors for dynamic control of metabolic pathways.